中国水稻科学 ›› 2014, Vol. 28 ›› Issue (1): 1-8.DOI: 10.3969/j.issn.1001-7216.2014.01.001

• 研究报告 •    下一篇

磷转运蛋白OsPT6在水稻武育粳7号苗期磷素吸收利用中的作用

张芳#,艾昊#,王丹凤,曹越,史书林,顾冕,孙淑斌*,徐国华   

  1. 南京农业大学 资源与环境科学学院, 南京210095;
  • 收稿日期:2013-02-03 修回日期:2013-07-04 出版日期:2014-01-10 发布日期:2014-01-10
  • 通讯作者: 孙淑斌*
  • 基金资助:

    国家自然科学基金资助项目(31172014, 30871582)。

Roles of OsPT6 on the Phosphate Uptake and Utilization in the Rice Seeding of Wuyujing 7

ZHANG Fang#, AI Hao#, WANG Danfeng, CAO Yue, SHI Shulin, GU Mian, SUN Shubin*, XU Guohua   

  1. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;
  • Received:2013-02-03 Revised:2013-07-04 Online:2014-01-10 Published:2014-01-10
  • Contact: SUN Shubin*,

摘要: 磷转运蛋白OsPT6为水稻Pht1家族成员之一,具有吸收和转运磷酸盐双重功能。以水稻高产品种武育粳7号的OsPT6超表达转基因材料为试材,研究磷转运蛋白OsPT6在武育粳7号磷素吸收利用中的作用。结果表明:1)OsPT6在武育粳7号的地下部表达丰度较高,同时在地下部和地上部该基因均受缺磷诱导表达量显著增加。2)OsPT6超表达后,转基因植株生长良好。正常供磷和低磷处理35 d后,OsPT6超表达植株地上部和地下部干物质量都显著增加。3)OsPT6超表达转基因材料在不同浓度磷素处理后,各组织全磷含量有所增加,其中营养器官尤为显著。

关键词: 水稻, 磷转运蛋白, 超表达

Abstract: As one of members of the Pht1 transporters family in rice,the phosphate transporter OsPT6 plays a broad role in Pi uptake, translocation and internal transport throughout the plant. With the OsPT6 overexpressed transgenic rice of  Wuyujing 7, a highyielding variety, we studied the roles of OsPT6 on the phosphate uptake and utilization in rice. The main results are the following: 1)OsPT6 is abundantly expressed in roots of Wuyujing 7. Furthermore, the expression levels of OsPT6 in shoots and roots are induced by phosphate deficiency. 2)The overexpression of OsPT6 can promote the growth of plants.  The dry weight of shoots and roots of the  OsPT6 overexpressed transgenic plants was significantly increased, in both sufficient and deficientPi conditions for 35 days. 3)The total P concentration increases in different tissues and organs of OsPT6 overexpressed transgenic plants, especially in vegetable organs, at different Pisupply levels.

Key words: rice, phosphate transporter, overexpression

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